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Application of Three Spectrometric Methods to Total Selenium Determination in Postmortem Material in a Case of Acute Selenium Compound Poisoning
Author(s) -
WietechaPosłuszny Renata,
Lech Teresa,
Kościelniak Paweł
Publication year - 2011
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2010.01660.x
Subject(s) - selenium , chemistry , environmental chemistry , chromatography , organic chemistry
Three spectrometric methods, that is, spectrofluorimetry (SF), atomic absorption spectrometry with electrothermal atomization (ET‐AAS), and atomic fluorescence spectrometry with hydride generation (HG‐AFS) were used for the determination of total selenium in biological samples taken from postmortem material in a case of acute selenium compound poisoning. The precision of the SF, ET‐AAS, and HG‐AFS methods (RSD, n = 10) was found to be in the ranges of 10.0–15.0, 3.0–6.0 and 1.0–1.5%, respectively, and the detection limit was 10.0, 4.0, and 0.1 μg/L of Se, respectively. In the case of HG‐AFS, the analytical procedure takes less time and is less laborious than the other methods considered. The obtained results show the usefulness of the HG‐AFS method as a supplementary analytical tool to the SF and ET‐AAS methods with respect to the determination of selenium as well as the possibility of using this method as a primary one in forensic toxicology practice.