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A Novel Method for ABO Genotyping Using a DNA Chip
Author(s) -
Watanabe Ken,
Ikegaya Hiroshi,
Hirayama Koichi,
Motani Hisako,
Iwase Hirotaro,
Kaneko Hiroto,
Fukushima Hisayo,
Akutsu Tomoko,
Sakurada Koichi
Publication year - 2011
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2010.01630.x
Subject(s) - genotyping , abo blood group system , polymerase chain reaction , biology , genetics , single nucleotide polymorphism , genotype , gene , microbiology and biotechnology , computational biology
ABO genotyping is often performed to identify the blood type of decomposed samples, which is difficult to be determined by a serological test. In this study, we developed a simple method for ABO genotyping using a DNA chip. In this method, polymerase chain reaction‐amplified and fluorescent‐labeled fragments in the ABO gene and primate‐specific D17Z1 were hybridized with DNA probes on a chip designed to detect single nucleotide polymorphisms (SNPs) in the ABO gene and part of the D17Z1 sequence. Using blood samples from 42 volunteers and 10 animal species, we investigated whether the chip could be used to detect SNPs in the ABO gene and the D17Z1 sequence. This method was then applied to various forensic samples, and it was confirmed that this method was suitable for the simultaneous analyses of ABO genotyping and species identification. This method fulfills the recent need for the development of rapid and convenient methods for criminal investigations.