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Full STR Profile of a 67‐Year‐Old Bone Found in a Fresh Water Lake
Author(s) -
Courts Cornelius,
Madea Burkhard
Publication year - 2011
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2010.01589.x
Subject(s) - dna extraction , microsatellite , dna , polymerase chain reaction , str analysis , extraction (chemistry) , chromatography , typing , biology , dna profiling , microbiology and biotechnology , chemistry , genetics , gene , allele
DNA extraction from and DNA typing of fresh water‐exposed aged bone specimens poses a challenging task and is not very well examined. This study presents a new method to extract typable DNA from such problematic bone specimens. The procedure comprises low‐heat drilling and cryogrinding, mild lysis conditions, and silica‐column‐based DNA cleaning. DNA quantity is assessed by quantitative PCR prior to short tandem repeat (STR) amplification. The procedure was employed with a 67‐year‐old tibia bone fragment recovered from a fresh water lake and succeeded to produce a full STR profile using the MPX‐SP1 and MPX‐SP2 mini‐STR kits and a partial profile with 12 successfully amplified STRs using the Identifiler STR kit. The new method for the extraction of DNA from aged fresh water‐exposed bone specimens presented herein was successfully applied to prepare DNA of sufficient quality and quantity to generate a full STR profile.