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A Simple Identification Method of Saliva by Detecting Streptococcus salivarius Using Loop‐Mediated Isothermal Amplification
Author(s) -
Nakanishi Hiroaki,
Ohmori Takeshi,
Hara Masaaki,
Takada Aya,
Shojo Hideki,
Adachi Noboru,
Saito Kazuyuki
Publication year - 2011
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2010.01579.x
Subject(s) - loop mediated isothermal amplification , streptococcus salivarius , saliva , chromatography , chemistry , biology , streptococcus , biochemistry , bacteria , genetics , dna
We previously reported that detection of Streptococcus salivarius is feasible for proving the presence of saliva in a forensic sample. Here, a simple and rapid method for the detection of S. salivarius in forensic samples was developed that uses loop‐mediated isothermal amplification (LAMP). The LAMP primer set was designed using S. salivarius ‐specific sequences of glucosyltransferase K. To simplify the procedure, the sample was prepared by boiling and mutanolysin treatment only, and the entire analytical process was completed within 2.5 h. The cut‐off value was set at 0.1 absorbance units, measured at 660 nm, upon termination of the reaction. S. salivarius was identified in all saliva samples, but was not detected in other body fluids or on the skin surface. Using this method, S. salivarius was successfully detected in various mock forensic samples. We therefore suggest that this approach is useful for the identification of saliva in forensic practice.