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Determining the Source of Equine Bloodstains by Dinucleotide Repeats *
Author(s) -
Chen JinWen,
Uboh Cornelius E.,
Soma Lawrence R.,
Li Xiaoqing,
Guan Fuyu,
You Youwen,
Liu Ying
Publication year - 2010
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2010.01466.x
Subject(s) - multiplex , suspect , biology , genetics , locus (genetics) , dna profiling , dna , gene , psychology , criminology
A novel multiplex of independent dinucleotide tandem repeat (DTR) loci was previously described that is capable of not only discriminating human and equine DNA, but of identifying a single equine source. We report a case in which a bloodstained syringe and two needles were found during inspection of a barn by inspectors of the Pennsylvania Racing Commissions. Using the multiplex and single‐locus detection, all 21 equine DTR markers were detected in a suspect horse and two evidence samples, indicating the evidence samples came from the suspect animal. Only six markers were detected in the third evidence sample because the volume of blood was limited. Following whole‐genome amplification and single‐locus PCR, the third evidence sample detected a total of 17 markers and the likelihood of identity (probability from suspect horse/probability from a random pacer) was 7.0 × 10 6 . The DTR multiplex has some technical limitations, but it is already practical for casework.