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Validation of the AMPFℓSTR ® MiniFiler TM PCR Amplification Kit for Use in Forensic Casework *
Author(s) -
Luce Coral,
Montpetit Shawn,
Gangitano David,
O’Donnell Patrick
Publication year - 2009
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2009.01099.x
Subject(s) - genotype , concordance , microbiology and biotechnology , locus (genetics) , dna profiling , polymerase chain reaction , biology , microsatellite , str analysis , genetics , allele , dna , gene
The AmpF ℓ STR ® MiniFiler TM PCR Amplification Kit is designed to genotype degraded and/or inhibited DNA samples when the AmpF ℓ STR ® Identifiler TM PCR Amplification Kit is incapable of generating a complete genetic profile. Validation experiments, following the SWGDAM guidelines, were designed to evaluate the performance of MiniFiler. Data obtained demonstrated that MiniFiler, when used in conjunction with Identifiler, provided an increased ability to obtain genetic profiles from challenged samples. The optimum template range was found to be between 0.2 and 0.6 ng, with 0.3 ng yielding the best results. Full concordance was achieved between the MiniFiler kit and Identifiler kit except in a single case of a null allele at locus D21S11. Numerous instances of severe heterozygous peak imbalance (<50%) were observed in single source samples amplified within the optimum range of input DNA suggesting that caution be taken when attempting to deduce component genotypes in a mixture.