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Canine Population Data Generated from a Multiplex STR Kit for Use in Forensic Casework *
Author(s) -
Kanthaswamy Sree,
Tom Bradley K.,
Mattila AnnaMaria,
Johnston Eric,
Dayton Melody,
Kinaga Jennifer,
JoyAlise Erickson Bethany,
Halverson Joy,
Fantin Dennis,
DeNise Sue,
Kou Alexander,
Malladi Venkat,
Satkoski Jessica,
Budowle Bruce,
Glenn Smith David,
Koskinen Mikko T.
Publication year - 2009
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2009.01080.x
Subject(s) - genotyping , multiplex , forensic science , breed , microsatellite , population , str multiplex system , biology , genetics , allele , locus (genetics) , dna profiling , evolutionary biology , genotype , medicine , dna , gene , environmental health
Canine biological specimens are often part of the physical evidence from crime scenes. Until now, there have been no validated canine‐specific forensic reagent kits available. A multiplex genotyping system, comprising 18 short tandem repeats (STRs) and a sex‐linked zinc finger locus for gender determination, was developed for generating population genetic data assessing the weight of canine forensic DNA profiles. Allele frequencies were estimated for 236 pedigreed and 431 mixed breed dogs residing in the U.S. Average random match probability is 1 in 2 × 10 33 using the regional database and 1 in 4 × 10 39 using the breed dataset. Each pedigreed population was genetically distinct and could be differentiated from the mixed breed dog population but genetic variation was not significantly correlated with geographic transition. Results herein support the use of the allele frequency data with the canine STR multiplex for conveying the significance of identity testing for forensic casework, parentage testing, and breed assignments.