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Simple and Sensitive Method for Identification of Human DNA by Allele‐Specific Polymerase Chain Reaction of FOXP2
Author(s) -
Hiroshige Kenichi,
Soejima Mikiko,
Nishioka Tomoki,
Kamimura Shigeo,
Koda Yoshiro
Publication year - 2009
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2009.01063.x
Subject(s) - polymerase chain reaction , biology , genomic dna , genetics , dna , microsatellite , foxp2 , inverse polymerase chain reaction , in silico pcr , gene , forensic identification , microbiology and biotechnology , primer dimer , dna sequencing , computational biology , allele , multiplex polymerase chain reaction , transcription factor
The forkhead box P2 ( FOXP2 ) gene is specifically involved in speech and language development in humans. The sequence is well conserved among many vertebrate species but has accumulated amino acid changes in the human lineage. The aim of this study was to develop a simple method to discriminate between human and nonhuman vertebrate DNA in forensic specimens by amplification of a human‐specific genomic region. In the present study, we designed an allele‐specific polymerase chain reaction (PCR) using primers to amplify smaller than 70‐bp regions of FOXP2 to identify DNA as being of human or nonhuman, including ape, origin. PCR amplification was also successfully performed using fluorescence‐labeled primers, and this method allows a single PCR reaction with a genomic DNA sample as small as 0.01 ng. This system also identified the presence of human DNA in two blood stains stored for 20 and 38 years. The results suggested the potential usefulness of FOXP2 as an identifier of human DNA in forensic samples.