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Thin Layer Chromatography/Fluorescence Detection of 3,4‐Methylenedioxy‐Methamphetamine and Related Compounds
Author(s) -
Kato Noriyuki,
Fujita Susumu,
Ohta Hikoto,
Fukuba Makoto,
Toriba Akira,
Hayakawa Kazuichi
Publication year - 2008
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2008.00870.x
Subject(s) - fluorescence , methylenedioxy , chemistry , sodium hypochlorite , chromatography , sodium hydroxide , detection limit , potassium hydroxide , thin layer chromatography , reagent , sodium , mdma , fluorescence spectroscopy , organic chemistry , medicine , alkyl , physics , halogen , quantum mechanics , pharmacology
A rapid and sensitive method for the detection of six methylenedioxylatedphenethylamines, 3,4‐methylenedioxymethamphetamine (MDMA); 3,4‐methylenedioxyamphetamine; 3,4‐methylenedioxyethylamphetamine; N ‐methyl‐1‐(3,4‐methylenedioxyphenyl)‐2‐butamine; N ‐methyl‐1‐(3,4‐methylenedioxyphenyl)‐3‐butamine; and 3,4‐methylenedioxydimethylamphetamine, by thin‐layer chromatography with fluorescence detection is proposed. These compounds form fluorophores on the developing plate following spraying with a reagent consisting of sodium hypochlorite, potassium hexacyanoferrate (III), and sodium hydroxide, and heating for 3 min at 100°C. Blue fluorescent spots were observed under ultraviolet light in a wavelength range of 250–400 nm. The detection limits for MDMA and the above related compounds were 50 ng. The proposed method was effectively applied to the detection of MDMA in urine samples.