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Quantifiler™ Observations of Relevance to Forensic Casework *
Author(s) -
Koukoulas Irene,
O’Toole Fiona E.,
Stringer Peta,
Van Oorschot Roland A. H.
Publication year - 2008
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2007.00616.x
Subject(s) - forensic science , dna , dna profiling , computational biology , relevance (law) , str analysis , chromatography , chemistry , biology , genetics , microsatellite , allele , political science , gene , law
The Quantifiler™ (QF) kit is regularly used by forensic scientists for DNA quantitation. We performed in‐house validation studies which revealed some interesting observations. The QF standard displayed a two‐fold difference between two different lot numbers which suggests that every standard should be tested prior to use. The Promega K562 DNA standard works well with the QF kit. c. 41% of samples that inhibited the internal PCR control (IPC) system within the QF kit still produced good Profiler Plus™ reactions. QIAquick ® was effective at removing inhibitors. The presence of dyes within casework samples were observed not to inhibit QF amplifications. Template DNA greater than 100 ng/μL appeared to inhibit the IPC. Close to identical concentration results were obtained when alternative analysis settings were used. These validation findings will assist DNA processes involved in forensic casework.