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Forensic Utility of the Mitochondrial Hypervariable Region 1 of Domestic Dogs, in Conjunction with Breed and Geographic Information *
Author(s) -
Himmelberger Andrea L.,
Spear Theresa F.,
Satkoski Jessica A.,
George Debra A.,
Garnica Wendy T.,
Malladi Venkat S.,
Smith David G.,
Webb Kristen M.,
Allard Marc W.,
Kanthaswamy Sreetharan
Publication year - 2008
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2007.00615.x
Subject(s) - haplotype , hypervariable region , mitochondrial dna , breed , genbank , biology , genetics , population , mtdna control region , genetic variation , donkey , evolutionary biology , genotype , gene , medicine , ecology , environmental health
The 608‐bp hypervariable region 1 (HV1) sequences from 36 local dogs were analyzed to characterize the population genetic structure of canid mitochondrial DNA (mtDNA). Sixteen haplotypes were identified. A 417‐bp segment of this sequence was compared with GenBank sequences from a geographically representative sample of 201 dogs, two coyotes, and two wolves. Sixty‐six haplotypes were identified including 62 found only in domestic dogs. Fourteen of these correspond to the 16 local haplotypes and were among the most frequent haplotypes. The local sample was judged to be representative of the much broader geographic sample. No correlation was observed between local haplotypes and the owner’s characterization of dog breed. A 60‐bp variation “hotspot” within the canid HV1 was identified as a potentially valuable molecular tool, particularly for assaying limited or degraded DNA samples.