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HPLC–DAD Determination of Mepivacaine in Cerebrospinal Fluid from a Fatal Case
Author(s) -
Nieddu Maria,
Boatto Gianpiero,
Serra Domenico,
Soro Aldo,
Lorenzoni Salvatore,
Lubinu Francesco
Publication year - 2007
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2007.00526.x
Subject(s) - mepivacaine , high performance liquid chromatography , chromatography , cerebrospinal fluid , chemistry , anesthesia , acetonitrile , medicine , bupivacaine
A fatal case involving mepivacaine‐induced epidural anesthesia is described. The pathological findings were typical of cardiac shock from ischemic origin. Cerebrospinal fluid (CSF) was obtained several hours after death and mepivacaine was identified by gas chromatography‐mass spectrometry (GC‐MS). Its concentration was determined by high performance liquid chromatography with diode array detection (HPLC–DAD). Extraction from CSF was performed by deproteinization with acetonitrile. The mepivacaine concentration in the sample was 264 μg/mL. Concentrations of mepivacaine in CSF following epidural anesthesia are not reported in literature to our knowledge. This is the first reported case of death in which the mepivacaine concentration in CSF has been determined.