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Typeability of AmpFlSTR SGM Plus Loci in Brain and Thyroid Gland Tissue Samples Incubated in Different Environments *
Author(s) -
NiemcunowiczJanica Anna,
Pepinski Witold,
Janica Jacek R.,
Skawronska Malgorzata,
J Jerzy Janica,
KocZorawska Ewa
Publication year - 2007
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2007.00465.x
Subject(s) - thyroid , pathology , biology , genotyping , brain tissue , genetics , anatomy , medicine , gene , genotype
Autolysis and putrefaction are crucial factors responsible for degradation of cells, tissues, and organs. Postmortem changes may assume different course depending on extrinsic and intrinsic conditions. The aim of the study was assessment of environmental effect on typeability of AmpFlSTR SGM Plus loci: D3S1358, VWA, D16S539, D2S1338, D81179, D21S11, D18S51, D19S433, TH01, FGA, and gender marker amelogenin. Brain and thyroid gland tissue specimens collected during autopsies of five persons aged 20–30 years were incubated at 21°C and 4°C in different environmental conditions. DNA was extracted by organic method from tissue samples collected in 7‐day intervals and subsequently typed using AmpFlSTR SGM Plus kit and ABI 310. A fast decrease in typeability rate was seen in specimens incubated in peat soil and in sand. Brain tissue samples were typeable in all AmpFlSTR SGM Plus loci within 126 days of incubation at 4°C. Faster DNA degradation was recorded in thyroid gland specimens. In samples with negative genotyping results, no DNA was found by fluorometric quantitiation.