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Real‐Time Polymerase Chain Reaction Quantification of Canine DNA *
Author(s) -
Evans Jeffrey J.,
Wictum Elizabeth J.,
Penedo M. Cecilia T.,
Kanthaswamy Sreetharan
Publication year - 2007
Publication title -
journal of forensic sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.715
H-Index - 96
eISSN - 1556-4029
pISSN - 0022-1198
DOI - 10.1111/j.1556-4029.2006.00305.x
Subject(s) - taqman , polymerase chain reaction , dna , real time polymerase chain reaction , biology , dna profiling , computational biology , digital polymerase chain reaction , primer dimer , genomic dna , microbiology and biotechnology , gene , multiplex polymerase chain reaction , genetics
The accurate quantification of target DNA is an important step in the short tandem repeat analysis of forensic biological samples. By utilizing quantification data to control the amount of template DNA in the polymerase chain reaction (PCR), forensic scientists can optimize testing and minimize the consumption of limited samples. The ability to identify and quantify target DNA in mixed‐species samples is crucial when it may be overwhelmed by nontarget DNA, as in cases of dog attack. We evaluated two quantitative real‐time PCR assays for dynamic range, species specificity, and inhibition by humic acid. While both assays proved to be highly sensitive and discriminating, the Melanocortin‐1 Receptor ( MC1R ) gene Taqman ® assay had the advantages of a shorter run time, greater efficiency, and safer reagents. In its application to forensic casework, the MC1R assay has been advantageous for quantifying dog DNA in a variety of mixed‐species samples and facilitating the successful profiling of individual dogs.