Premium
Determination of the Effect of In Vitro Time, Temperature, and Tourniquet Use on Whole Blood Venous Point‐of‐care Lactate Concentrations
Author(s) -
Jones Alan E.,
Leonard Matthew M.,
HernandezNino Jackeline,
Kline Jeffrey A.
Publication year - 2007
Publication title -
academic emergency medicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.221
H-Index - 124
eISSN - 1553-2712
pISSN - 1069-6563
DOI - 10.1111/j.1553-2712.2007.tb01840.x
Subject(s) - medicine , tourniquet , venipuncture , venous blood , anesthesia , blood lactate , randomized controlled trial , whole blood , analysis of variance , surgery , heart rate , blood pressure
Objectives: The authors sought to determine the effect of in vitro time, temperature, and removable tourniquet use on changes in venous point‐of‐care lactate concentrations. Methods: This was a prospective randomized trial on healthy volunteers. Subjects were randomized to one of three groups: group 1 had venous lactate concentrations measured on blood drawn without a tourniquet and the sample placed in ice (−1°C), group 2 had lactate concentrations measured on blood drawn without a tourniquet and the sample left at 23°C, and group 3 had lactate concentrations measured on blood drawn with a tourniquet placed 5 minutes before venipuncture and the sample placed in ice (−1°C). Lactate concentrations were measured on a point‐of‐care device at time 0, 3, 6, 9, 12, and 15 minutes in all three groups. Mean lactate concentrations were analyzed using a two‐way repeated‐measures analysis of variance. Results: Eighty subjects were randomized, with complete data available in 63 (21 per group). Over the 15‐minute period, lactate concentrations