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Nucleus‐encoded mRNA s for Chloroplast Proteins GapA , PetA , and PsbO are Trans ‐spliced in the Flagellate Euglena gracilis Irrespective of Light and Plastid Function
Author(s) -
MateášikováKováčová Bianka,
Vesteg Matej,
Drahovská Hana,
Záhonová Kristína,
Vacula Rostislav,
Krajčovič Juraj
Publication year - 2012
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2012.00634.x
Subject(s) - plastid , euglena gracilis , biology , chloroplast , flagellate , euglena , polyadenylation , cytochrome f , gene , photosystem i , messenger rna , botany , genetics
Euglena gracilis is a fresh‐water flagellate possessing secondary chloroplasts of green algal origin. In contrast with organisms possessing primary plastids, mRNA levels of nucleus‐encoded genes for chloroplast proteins in E. gracilis depend on neither light nor plastid function. However, it remains unknown, if all these mRNA s are trans ‐spliced and possess spliced leader sequence at the 5′‐end and if trans ‐splicing depends on light or functional plastids. This study revealed that polyadenylated mRNA s encoding the chloroplast proteins glyceraldehyde‐3‐phosphate dehydrogenase ( GapA ), cytochrome f ( PetA ), and subunit O of photosystem II ( PsbO ) are trans ‐spliced irrespective of light or plastid function.