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Molecular and Morphological Characterization of a Poorly Known Marine Ciliate, Myoschiston duplicatum Precht, [Precht, H., 1935]: Implications for Phylogenetic Relationships between Three Morphologically Similar Genera — Zoothamnium , Myoschiston, and Zoothamnopsis (Ciliophora, Peritrichia, Zoothamniidae)
Author(s) -
Sun Ping,
Xu Dapeng,
Clamp John C.,
Shin Mann Kyoon
Publication year - 2012
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2011.00609.x
Subject(s) - biology , ciliate , phylogenetic tree , evolutionary biology , zoology , phylogenetics , ecology , genetics , gene
We studied the morphology and molecular phylogeny of M yoschiston duplicatum , a peritrich ciliate that has been recorded as an epibiont of crustaceans, but which we also identified on marine algae from K orea. The important morphological characteristics revealed by silver staining of M yoschiston species have not been described because they are rarely collected. Using morphological methods, we redescribed the type species of the genus, M yoschiston duplicatum, and provided an improved diagnosis of M yoschiston . In addition, the coding regions for nuclear small subunit ( SSU ) rRNA and internal transcribed spacer 1‐5.8S‐internal transcribed spacer 2 sequences were sequenced. Phylogenetic analyses that included available SSU rDNA sequences of peritrichs from GenBank strongly supported a position of M . duplicatum within the family Z oothamniidae. In addition, phylogenetic analyses were performed with single datasets ( ITS 1‐5.8S‐ ITS 2) and combined datasets ( SSU rDNA + ITS 1‐5.8S‐ ITS 2) to explore further the phylogenetic relationship in the family Z oothamniidae between the three morphologically similar genera— Z oothamnium , M yoschiston, and Z oothamnopsis .