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In Vitro Dual Culture of Polymyxa betae in Agrobacterium rhizogenes Transformed Sugar Beet Hairy Roots in Liquid Media
Author(s) -
DESOIGNIES NICOLAS,
LEGREVE ANNE
Publication year - 2011
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2011.00563.x
Subject(s) - biology , agrobacterium , sugar beet , liquid medium , botany , liquid culture , in vitro , horticulture , transformation (genetics) , chemistry , biochemistry , chromatography , gene
.Polymyxa betae is a soil‐borne protist and an obligate parasite of sugar beet that transmits the beet necrotic yellow vein virus . Sugar beet hairy roots, transformed by Agrobacterium rhizogenes , were inoculated with surface‐sterilized root fragments infected by P. betae . After 10 wk in a liquid medium, typical structures of P. betae were observed in this in vitro system. This first in vitro culture of P. betae in liquid medium will contribute to a better understanding of this protist's biology through providing a way to conserve and produce purified isolates of the protist.