Development of a Nested PCR for Environmental Detection of the Pathogenic Free‐Living Amoeba  Balamuthia mandrillaris | Zendy

AHMAD ARINE F. | Zendy; ANDREW PETER W. | Zendy; KILVINGTON SIMON | Zendy

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Development of a Nested PCR for Environmental Detection of the Pathogenic Free‐Living Amoeba Balamuthia mandrillaris

Author(s) -

AHMAD ARINE F.,

ANDREW PETER W.,

KILVINGTON SIMON

Publication year - 2011

Publication title -

journal of eukaryotic microbiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.067

H-Index - 77

eISSN - 1550-7408

pISSN - 1066-5234

DOI - 10.1111/j.1550-7408.2011.00541.x

Subject(s) - biology , nested polymerase chain reaction , amoeba (genus) , ecology , environmental dna , zoology , microbiology and biotechnology , polymerase chain reaction , genetics , gene , biodiversity

. A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk.

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