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Development of a Nested PCR for Environmental Detection of the Pathogenic Free‐Living Amoeba Balamuthia mandrillaris
Author(s) -
AHMAD ARINE F.,
ANDREW PETER W.,
KILVINGTON SIMON
Publication year - 2011
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2011.00541.x
Subject(s) - biology , nested polymerase chain reaction , amoeba (genus) , ecology , environmental dna , zoology , microbiology and biotechnology , polymerase chain reaction , genetics , gene , biodiversity
. A DNA extraction and nested PCR method for detecting the pathogenic amoeba Balamuthia mandrillaris from the environment was developed. Sixteen of 17 Californian soil samples were positive compared with 0/44 from the United Kingdom. This approach will enable a greater understanding of B. mandrillaris ecology, geographic distribution, and public health risk.