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Identification of Two Spliced Leader RNA Transcripts from Perkinsus marinus
Author(s) -
HEARNE JENNIFER L.,
PITULA JOSEPH S.
Publication year - 2011
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2011.00538.x
Subject(s) - biology , rna , complementary dna , microbiology and biotechnology , transcription (linguistics) , genetics , consensus sequence , messenger rna , nucleic acid sequence , rna splicing , gene , nucleotide , base sequence , linguistics , philosophy
. Spliced leader (SL) variants are present in a number of mRNAs from Perkinsus marinus . Three different SLs of 22 nucleotides (nt) in length were previously reported, with a consensus sequence of (DCCGUAGCCAUYUUGGCUCAAG). A truncated 21 nt SL, with an (A) at nt‐1 and a (U) deletion at nt‐13, has also been reported. Here, we report an additional 21 nt SL variant with (G) at nt‐1. Using cDNA analysis, a full‐length SL RNA transcript was identified for both 21 nt SLs (SL2[A] and SL2[G]). This transcript is 81 nt in length and contains a conserved transcription termination sequence present in closely related dinoflagellates.