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Phylogenetic Analysis of Complete rRNA Gene Sequence of Nosema philosamiae Isolated from the Lepidopteran Philosamia cynthia ricini
Author(s) -
ZHU FENG,
SHEN ZHONGYUAN,
XU XIAOFANG,
TAO HENGPING,
DONG SHINAN,
TANG XUDONG,
XU LI
Publication year - 2010
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2010.00475.x
Subject(s) - biology , nosema , phylogenetic tree , ribosomal rna , gene , genetics , phylogenetics , sequence analysis , ribosomal dna , polymerase chain reaction , microsporidia , botany , spore
. The microsporidian Nosema philosamiae is a pathogen that infects the eri‐silkworm Philosamia cynthia ricini . The complete sequence of rRNA gene (4,314 bp) was obtained by polymerase chain reaction amplification with specific primers and sequencing. The sequence analysis showed that the organization of the rRNA of N. philosamiae was similar to the pattern of Nosema bombycis . Phylogenetic analysis of rRNA gene sequences revealed that N. philosamiae had a close relationship with other Nosema species, confirming that N. philosamiae is correctly assigned to the genus Nosema .