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Characterization of Genotypes of Enterocytozoon bieneusi in Immunosuppressed and Immunocompetent Patient Groups
Author(s) -
TEN HOVE ROBERT J.,
VAN LIESHOUT LISETTE,
BEADSWORTH MIKE B. J.,
PEREZ M. ARANTZA,
SPEE KLAARTJE,
CLAAS ERIC C.J.,
VERWEIJ JACO J.
Publication year - 2009
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2009.00393.x
Subject(s) - biology , genotype , enterocytozoon bieneusi , internal transcribed spacer , phylogenetic tree , clade , phylogenetics , genetics , virology , gene
ABSTRACT. A retrospective phylogenetic analysis was performed on isolates of Enterocytozoon bieneusi to characterize the genotypes in different patient cohorts. Fifty‐seven isolates, collected from patients living in Malawi and the Netherlands, were classified by age and immune status of the hosts. Sequence analysis of the internal transcribed spacer (ITS) region identified 16 genotypes; nine have not previously been described. Genotypes K and D were most prevalent among patient groups, whereas genotype C was restricted to transplantation patients receiving immunosupressives and genotype B showed a predisposition toward patients living with HIV/AIDS. Different genotypes showed more dispersion among isolates from Malawi compared with those from the Netherlands. A constructed map estimating the genealogy of the ITS region reveals a dynamic evolutionary process between the genotypes.