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Distinct Localization Patterns of Two Putative Mitochondrial Proteins in the Microsporidian Encephalitozoon cuniculi
Author(s) -
WILLIAMS BRYONY A. P.,
CALI ANN,
TAKVORIAN PETER M.,
KEELING PATRICK J.
Publication year - 2008
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2008.00315.x
Subject(s) - encephalitozoon cuniculi , biology , mitochondrion , microsporidia , organelle , cytoplasm , mitochondrial dna , microbiology and biotechnology , biochemistry , gene , spore
. Microsporidia were once considered amitochondriate, but have now been found to retain relict mitochondria called mitosomes. These organelles have been identified by immunolocalization in Trachipleistophora hominis , whereas most data on function have been inferred from the presence of mitochondrial protein‐encoding sequences in the genome of Encephalitozoon cuniculi . Here we describe the localization of two such enzymes in E. cuniculi cells. Immunofluorescent localization of ferredoxin involved in mitochondrial iron–sulfur cluster assembly reveals a punctate distribution as expected for mitochondria. In contrast, localization of mitochondrial glycerol‐3‐phosphate dehydrogenase suggests a cytoplasmic distribution in E. cuniculi and possible relocalization of this typically mitochondrial protein.