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Microheterogeneity and Coevolution: An Examination of rDNA Sequence Characteristics in Neoparamoeba pemaquidensis and Its Prokinetoplastid Endosymbiont
Author(s) -
CARAGUEL CHARLES G. B.,
O'KELLY CHARLES J.,
LEGENDRE PIERRE,
FRASCA SALVATORE,
GAST REBECCA J.,
DESPRÉS BÉATRİCE M.,
CAWTHORN RICHARD J.,
GREENWOOD SPENCER J.
Publication year - 2007
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2007.00281.x
Subject(s) - biology , coevolution , evolutionary biology , sequence (biology) , genetics
.Neoparamoeba pemaquidensis , the etiological agent of amoebic gill disease, has shown surprising sequence variability among different copies of the 18S ribosomal RNA gene within an isolate. This intra‐genomic microheterogeneity was confirmed and extended to an analysis of the internal transcribed spacer (ITS) region. High levels of intra‐genomic nucleotide diversity (Pi=0.0201–0.0313) were found among sequenced ITS regions from individual host amoeba isolates. In contrast, the ITS region of its endosymbiont revealed significantly lower levels of intra‐genomic nucleotide diversity (Pi=0.0028–0.0056) compared with the host N. pemaquidensis . Phylogenetic and ParaFit coevolution analyses involving N. pemaquidensis isolates and their respective endosymbionts confirmed a significant coevolutionary relationship between the two protists. The observation of non‐shared microheterogeneity and coevolution emphasizes the complexity of the interactions between N. pemaquidensis and its obligate endosymbiont.