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Ultrastructural and Phylogenetic Studies on Blastocystis Isolates from Cockroaches
Author(s) -
YOSHIKAWA HISAO,
WU ZHILIANG,
HOWE JOSEPHINE,
HASHIMOTO TETSUO,
GEOKCHOO NG,
TAN KEVIN S. W.
Publication year - 2007
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2006.00141.x
Subject(s) - blastocystis , biology , clade , phylogenetic tree , phylogenetics , zoology , cockroach , ribosomal rna , evolutionary biology , genetics , gene , microbiology and biotechnology , ecology , feces
. Four Blastocystis isolates from cockroaches were established and these isolates were morphologically confirmed as Blastocystis organisms by light and/or electron microscopy. As these isolates were morphologically indistinguishable from Blastocystis isolated from other animals, phylogenetic analyses were conducted using their small subunit ribosomal RNA genes. A analyses of these sequences with previously reported ones that had been classified into nine Blastocystis clades indicated the presence of a new clade that comprised only Blastocystis organisms from cockroaches (clade X). A clade comprised of amphibian and reptilian Blastocystis organisms (clade IX) was located at the basal position of the Blastocystis tree together with the common ancestor of Proteromonas and Protoopalina , clade X emerged after the divergences of these two basal clades and its branching position was clearly supported by bootstrap analysis.