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Complete Sequence and Gene Organization of the Nosema spodopterae rRNA Gene
Author(s) -
Tsai ShuJen,
Huang WeiFone,
Wang ChungHsiung
Publication year - 2005
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2005.3291rr.x
Subject(s) - biology , nosema , ribosomal rna , internal transcribed spacer , genetics , gene , spacer dna , 18s ribosomal rna , 5s ribosomal rna , microsporidia , botany , spore
. By sequencing the entire ribosomal RNA (rRNA) gene of Nosema spodopterae , we show here that its gene organization follows a pattern similar to the Nosema type species, Nosema bombycis , i.e. 5′‐large subunit rRNA (2,497 bp)‐internal transcribed spacer (185 bp)‐small subunit rRNA (1,232 bp)‐intergenic spacer (277 bp)‐5S rRNA (114 bp)‐3′. Gene sequences and the secondary structures of large subunit rRNA, small subunit rRNA, and 5S rRNA are compared with the known corresponding sequences and structures of closely related microsporidia. The results suggest that the Nosema genus may be heterogeneous and that the rRNA gene organization may be a useful characteristic for determining which species are closely related to the type species.