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Localization by Indirect Immunofluorescence of Tetrin, Actin, and Centrin to the Oral Apparatus and Buccal Cavity of the Macrostomal Form of Tetrahymena vorax
Author(s) -
McLAUGHLIN NEIL B.,
BUHSE HOWARD E.
Publication year - 2004
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2004.tb00556.x
Subject(s) - biology , cytoskeleton , cytoplasm , actin , endoplasmic reticulum , microbiology and biotechnology , tetrahymena , protein filament , immunofluorescence , indirect immunofluorescence , antibody , cell , biochemistry , immunology
. We have taken advantage of the size of the macrostomal oral apparatus of Tetrahymena vorax to investigate the immunofluorescent localization of three cytoskeletal proteins—tetrin, actin, and centrin. Tetrin and actin antibodies co‐localize to cross‐connectives that anchor the membranelles. These antibodies also recognize the coarse filamentous reticulum, a filament associated with the undulating membrane. Actin‐specific localization extends beyond the coarse filamentous reticulum‐undulating membrane complex into a region called the specialized cytoplasm. A centrin antibody localizes to the fine filamentous reticulum which, along with micro‐tubules of the oral ribs, circumscribes the cytostomal opening. Models of phagocytic contraction based on these data are presented.