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Inhibition of Vorticetta microstoma Stalk Formation by Wheat Germ Agglutinin
Author(s) -
BRAMUCCI MICHAEL G.,
NAGARAJAN VASANTHA
Publication year - 2004
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2004.tb00389.x
Subject(s) - stalk , wheat germ agglutinin , biology , concanavalin a , agglutinin , germ , ultrastructure , biochemistry , microbiology and biotechnology , anatomy , lectin , in vitro , horticulture
Fluorescently labeled conjugates of wheat germ agglutinin and concanavalin A stained the contractile stalk but not the cell body of Vorticetta microstoma trophonts. Binding of the fluorescent conjugants did not noticeably alter the activity of the trophonts. However, unconjugated wheat germ agglutinin prevented free swimming telotrochs from adhering to a glass surface and deploying a contractile stalk during differentiation into trophonts. These observations indicated that the stalk, the material that binds the stalk to surfaces, and the precursors for these components have saccharide residues in common.