Premium
Cell Death Mechanisms in the Human Opportunistic Pathogen Candida albicans
Author(s) -
Lemar Katey M.,
Müller Carsten T.,
Plummer Susan,
Lloyd David
Publication year - 2003
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2003.tb00687.x
Subject(s) - propidium iodide , programmed cell death , candida albicans , biology , oxidative stress , apoptosis , reactive oxygen species , flow cytometry , annexin , mitochondrion , population , cell , microbiology and biotechnology , biochemistry , immunology , medicine , environmental health
. Difficulties arising during chemotherapy of Candida albicans necessitate novel chemotherapeutic strategies. Garlic extract and two of its constituents, diallyl disulphide and allyl alcohol, are potentially useful anti‐candidal agents. Flow Cytometry has been used to measure the population distributions of apoptotic/necrotic cell death using annexin V‐FITC/propidium iodide and oxidative stress dichlorodihydrofluorescein. Candicidal mechanisms may be due to programmed cell death induced by oxidative stress, mediated by the generation of reactive oxygen species or alternatively by the depletion of cellular thiols, which normally act as redox buffer systems for defence. We suggest that mechanisms that these anti‐candidal agents have in common is the triggering some of the characteristics of apoptotic cell death.