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Immunocompetent Infants as a Human Reservoir for Pneumocystis jirovecii: Rapid Screening by Non‐Invasive Sampling and Real‐Time PCR at the Mitochondria1 Large Subunit rRNA Gene
Author(s) -
TOTET ANNE,
MELIANI; LEILA,
LACUBE; PHILIPPE,
PAUTARD; JEAN CLAUDE,
RACCURT CHRISTIAN,
ROUX PATRICIA,
NEVEZ GILLES
Publication year - 2003
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2003.tb00678.x
Subject(s) - biology , pneumocystis jirovecii , ribosomal rna , locus (genetics) , real time polymerase chain reaction , gene , virology , genetics , human immunodeficiency virus (hiv)
. We tested a real‐time PCR assay targeting the Pneumocystis jirovecii mitochondria1 large subunit rRNA gene on 240 archival nasopharyngeal aspirates from non‐immunosuppressed infants. The sensitivity of this assay appeared close to that of a conventional nested‐PCR assay targeting the same locus. Because of its one‐step procedure, and its sensitivity and rapidity, the real‐time PCR assay is particularly suitable for screening individuals parasitized by P. jirovecii within large populations.