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Identification of a New Microsporidian Parasite Related to Vittaforma corneae in HIV‐Positive and HIV‐Negative Patients from Portugal
Author(s) -
SULAIMAN IRSHAD M.,
MATOS OLGA,
LOBO MARIA L.,
XIAO LIHUA
Publication year - 2003
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2003.tb00641.x
Subject(s) - biology , microsporidia , enterocytozoon bieneusi , parasite hosting , microsporidiosis , phylogenetic tree , virology , ribosomal rna , homology (biology) , zoology , genetics , gene , microbiology and biotechnology , spore , world wide web , computer science
. Fecal samples from 22 HIV‐positive and 3 HIV‐negative patients from Portugal with symptomatic diarrhea were diagnosed positive for microsporidia by microscopy, with most parasites detected significantly bigger than Enterocytozoon bieneusi and Encephalitozoon spp. Sequence characterization of the small subunit (SSU) rRNA gene identified a microsporidian parasite with 96% homology to two published Vittaforma corneae sequences. Phylogenetic analysis confirmed the genetic relatedness of this new microsporidian parasite to Vittaforma corneae as well as Cystosporogenes operophterae. Results of the study demonstrate the presence of a new human‐pathogenic microsporidian species, which is responsible for significant number of infections in HIV‐positive and HIV‐negative patients in Portugal.