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Cryptosporidium Species and Genotypes in HIV‐Positive Patients in Lima, Peru
Author(s) -
Cama Vitaliano A.,
Bern Caryn,
Sulaiman I.M.,
Gilman Robert H.,
Ticona Eduardo,
Vivar Aldo,
Kawai Vivian,
Vargas Daniel,
Zhou Ling,
Xiao Lihua
Publication year - 2003
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2003.tb00620.x
Subject(s) - cryptosporidium , biology , genotype , cryptosporidium parvum , restriction fragment length polymorphism , felis , veterinary medicine , microbiology and biotechnology , ribosomal rna , virology , feces , genetics , medicine , cats , gene
Cryptosporidium parasites from a cross‐sectional study conducted in two national hospitals in Lima, Peru were genetically characterized to deteimine the diversity of Cryptosporidium spp. in HIV‐positive people. A total of 2,672 patients participated in this study and provided 13,937 specimens. Cryptosporidium oocysts were detected by microscopy in 354 (13.3%) of the patients. Analysis of 951 Cryptosporidium ‐ positive specimens from 300 patients using a small subunit rRNA‐based PCR‐RFLP tool identified 6 genotypes; Cryptosporidium hominis was the species most frequently detected (67.5%), followed by C. meleagridis (12.6%) and C. parvum (11.3%). Cryptosporidium canis (4.0%), C. felis (3.3%), and Cryptosporidium pig genotype (0.5%) were also found. These findings indicate that C. hominis is the predominant species in Peruvian HIV‐positive persons, and that zoonotic Cryptosporidium spp. account for about 30% of cryptosporidiosis in these patients.