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Molecular Cloning and Sequencing of the Merozoite Surface Antigen 2 Gene from Plasmodium falciparum Strain FCC‐l/HN and Expression of the Gene in Mycobacteria 1
Author(s) -
ZHENG CHUNFU,
XIE PEIMEI,
CHEN YATANG
Publication year - 2003
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2003.tb00249.x
Subject(s) - biology , virology , plasmodium falciparum , mycobacterium bovis , merozoite surface protein , malaria vaccine , recombinant dna , mycobacterium tuberculosis , antigen , tuberculosis vaccines , plasmid , electroporation , microbiology and biotechnology , gene , esat 6 , attenuated vaccine , tuberculosis , malaria , genetics , virulence , immunology , medicine , pathology
. Strain bacillus Calmette‐Guerin (BCG) of Mycobacterium bovis has been used as a live bacterial vaccine to immunize more than 3 billion people against tuberculosis. In an attempt to use this vaccine strain as a vehicle for protective antigens, the gene encoding merozoite surface antigen 2 (MSA2) was amplified from strain FCC‐l/HN Plasmodium falciparum genome, sequenced, and expressed in M. bovis BCG under the control of an expression cassette carrying the promoter of heat shock protein 70 (HSP70) from Mycobacterium tuberculosis . The recombinant shuttle plasmid pBCG/MSA2 was introduced into mycobacteria by electroporation, and the recombinant mycobacteria harboring pBCG/MSA2 could be induced by heating to express MSA2; the molecular mass of recombinant MSA2 was about 31 kDa. This first report of expression of the full‐length P. falciparum MSA2 gene in BCG provides evidence for use of the HSP70 promoter in expressing a foreign gene in BCG and in development of BCG as a multivalent vectoral vaccine for malaria.