Premium
Detection of Naegleria sp. in a Thermal, Acidic Stream in Yellowstone National Park
Author(s) -
SBEEBAN KATHY B.,
FERRIS MICHAEL J.,
BENSON JOAN M.
Publication year - 2003
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2003.tb00132.x
Subject(s) - naegleria , biology , internal transcribed spacer , 18s ribosomal rna , ribosomal rna , library , national park , naegleria fowleri , clone (java method) , 16s ribosomal rna , protozoa , taxonomy (biology) , zoology , gene , microbiology and biotechnology , ecology , genetics , virology , meningoencephalitis
. An initial survey of sequences of PCR‐amplified portions of the 18S rRNA genes from a community DNA clone library, prepared from an algal mat in a thermal, acidic stream in Yellowstone National Park, WY, USA, revealed among other sequences, several that matched Vahlkampfia . This finding prompted further investigation using primers specific for Naegleria . Sequences from a subsequent DNA clone library, prepared from the 5.8S rRNA gene and the adjacent internal transcribed spacer (ITS) regions of the rRNA, closely matched Naegleria and formed an independent lineage within a clade containing Naegleria sturti and Naegleria niuginiensis . The sequences may represent a new Naegleria species.