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Phylogenetic Position of Blastocystis hominis and of Stramenopiles Inferred from Multiple Molecular Sequence Data
Author(s) -
ARISUE NOBUKO,
HASHIMOTO TETSUO,
YOSHIKAWA HISAO,
NAKAMURA YOSHIfflRO,
NAKAMURA GEN,
NAKAMURA FUMINORI,
YANO TAKAAKI,
HASEGAWA MASAMI
Publication year - 2002
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2002.tb00339.x
Subject(s) - biology , blastocystis , phylogenetic tree , ribosomal rna , phylogenetics , genetics , protein subunit , ribosome , eukaryotic small ribosomal subunit , rna , microbiology and biotechnology , gene , feces
.Blastocystis hominis , a parasite of the human intestine, has recently been positioned within Stramenopiles by the small subunit rRNA phylogeny. To further confirm its phylogenetic position using multiple molecular sequence data, we determined the nucleotide sequences putatively encoding small subunit ribosomal RNA, cytosolic‐type 70‐kDa heat shock protein, translation elongation factor 2, and the non‐catalytic ‘B’ subunit of vacuolar ATPase of B. hominis (HE87–1 strain). Moreover, we determined the translation elongation factor 2 sequence of an apicomplexan parasite, Plasmodium falciparum , that belongs to alveolates. The maximum likelihood analyses of small subunit rRNA and cytosolic‐type 70‐kDa heat shock protein clearly demonstrated that B. hominis (HE87–1 strain) is positioned within Stramenopiles, being congruent with the previous small subunit rRNA analysis, including the sequences of B. hominis (Nand strain) and a Blastocystis isolate from guinea pig. Although no clear resolution among major eukaryotic groups was obtained by the individual phytogenies based on the four molecules analyzed here, a combined analysis of various molecules, including these, clearly indicated that Blatocystis /stramenopiles are the closest relatives of alveolates.