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Invasion of Different Cell Types by Sporozoites of Eimeria Species andEffects of Monoclonal Antibody 1209‐C2 on Invasion of Cells by Sporozoitesof Several Apicomplexan Parasites
Author(s) -
AUGUSTINE P. C.
Publication year - 2001
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2001.tb00300.x
Subject(s) - eimeria , biology , monoclonal antibody , baby hamster kidney cell , toxoplasma gondii , gentamicin protection assay , protozoa , microbiology and biotechnology , virology , monoclonal , antigen , cryptosporidium parvum , apicomplexa , cell , cell culture , antibody , immunology , biochemistry , protozoal disease , western blot , virus , malaria , gene , genetics
. Sporozoites of avian Eimeria species differed markedly in their ability to invade cells in vitro. Invasion by E. tenella and E. adenoeides was significantly greater in baby hamster kidney (BHK) and chicken cecal cell (CC) cultures than in primary chicken(PCK) or turkey kidney (PTK) cell cultures. Moreover, invasion of BHK cell cultures by E. adenoeides was significantly greater thanthat of other Eimeria species, and invasion by E. acervulina Sporozoites was significantly lower. Monoclonal antibody 1209‐C2 (MAb1209‐C2) reacted by immunofluorescent labeling (IFA) with refractile bodies of Sporozoites of 5 species of Eimeria and Caryosporabigenetica , but not with Sporozoites of Toxoplasma gondii, Hammondia hammondi , or Cryptosporidium parvum , which have no refractilebodies. The MAb also cross‐reacted with formalin‐fixed BHK, CC, turkey cecal (TC) cells, and PTK. Pretreatment of BHK cells withMAb 1209‐C2 significantly reduced invasion of the cells by Sporozoites of E. tenella, E. acervulina, E. meleagrimitis , and C. bigenetica , but did not alter invasion by T. gondii, C. parvum , or H. hammondia. Apparently, reactivity of MAB1209‐C2 with the Sporozoites wasrequired for inhibition of invasion despite the fact that the inhibition resulted from pre‐treatment of the host cell. Conversely, althoughMAb 1209‐C2 also reacted moderately with PTK and TC cells, pre‐treatment of these cell cultures with the MAb did not inhibit invasionby either MAB 1209‐C2‐reactive or ‐nonreactive parasites. Collectively, the data indicated that refractile body antigens of Sporozoitesof Eimeria and Caryospora , which are recognized by MAb 1209‐C2, may function in cellular invasion, but also suggest that cellularinvasion is probably not mediated by interactions between the conserved epitopes in sporozoites and cultured host cells that arerecognized by the MAb.