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Correlation Between In Vitro and In Vivo Infectivity of Leishmania infantum Clones
Author(s) -
MÉNDEZ SUSANA,
FERNÁNDEZPÉREZ FRANCISCO JOSÉ,
SANTÍN MÓNICA,
FUENTE CONCEPCIÓN,
CUQUERELLA MONTSERRAT,
GÓMEZMUÑOZ MARÍA TERESA,
ALUNDA JOSÉ MARÍA
Publication year - 2001
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2001.tb00200.x
Subject(s) - infectivity , leishmania infantum , biology , in vitro , in vivo , leishmania , strain (injury) , microbiology and biotechnology , polyclonal antibodies , leishmaniasis , virology , visceral leishmaniasis , immunology , genetics , parasite hosting , virus , antigen , anatomy , world wide web , computer science
. Eleven clones of a single strain of Leishmania infantum (MCAN/ES/88/ISS441, Doba) were analyzed for biological behavior in vivo and in vitro. Different clones showed differences in growth dependent upon the two culture media employed. All clones displayed only slight differences in H 2 O 2 and NaNO 2 sensitivity compared to the original strain, whereas in vitro infectivity for mouse peritoneal macrophages differed significantly among the clones. In vivo infections in hamsters correlated strongly with in vitro infectivity. The phenotypic differences found suggest a polyclonal structure for the Leishmania infantum strain studied.