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Programmed Translational Frameshifting Is Likely Required for Expressions of Genes Encoding Putative Nuclear Protein Kinases of the Ciliate Euplotes octocarinatus
Author(s) -
TAN MING,
LIANG AIHUA,
BRÜNENNIEWELER CLAUDIA,
HECKMANN KLAUS
Publication year - 2001
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2001.tb00193.x
Subject(s) - biology , translational frameshift , kinase , frameshift mutation , gene , protein serine threonine kinases , genetics , serine , grb10 , nuclear export signal , microbiology and biotechnology , protein kinase a , phosphorylation , rna , mutation , insulin receptor , insulin resistance , insulin , endocrinology
. Three macronuclear genes encoding putative nuclear protein kinases of the ciliate Euplotes octocarinatus syngen I were isolated and sequenced. All three deduced gene products share significant properties with a group of recently identified nuclear serine/ threonine protein kinases named Ndr. The three predicted proteins contain the twelve conserved catalytic subdomains of protein kinases and 22 near universally‐conserved amino acids residues that are characteristic of serine/threonine protein kinases. In addition, there is an—30 amino acid‐peptide insertion between subdomains VII and VIII that contains a potential nuclear localization signal. Sequence analysis suggests that expression of the Eondr2 gene requires a +1 programmed translational frameshift for its translation. Comparison of the deduced EoNdr2 with other known Ndr protein kinases implies that a +1 ribosomal frameshift occurs at the motif AAATAA.