Premium
Archaebacterial Relationships of the Phosphoenolpyruvate Carboxykinase Gene Reveal Mosaicism of Giardia intestinalis Core Metabolism
Author(s) -
SUGURI SETSUO,
HENZE KATRIN,
SÁNCHEZ LIDYA B.,
MOORE DOROTHY V.,
MÜLLER MIKLÓS
Publication year - 2001
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2001.tb00184.x
Subject(s) - biology , phosphoenolpyruvate carboxykinase , giardia , giardia lamblia , gene , protist , enzyme , biochemistry , genetics , operon , escherichia coli
. A gene encoding a putative GTP‐specific phosphoenolpyruvate carboxykinase has been cloned and sequenced from the type I amitochondriate protist Giardia intestinalis. The deduced amino acid sequence is related most closely to homologs from hyper‐thermophilic archaebacteria and only more distantly to homologs from Eubacteria and Metazoa. Most enzymes of Giardia core metabolism, however, are related more closely to eubacterial and metazoan homologs. An archaebacterial relationship has been noted previously for the unusual acetyl‐CoA synthetase (ADP‐forming) of this organism. The results suggest that phosphoenolpyruvate carboxykinase and acetyl‐CoA synthetase have been acquired from different sources than most enzymes of Giardia core metabolism.