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In Vivo and In Vitro Development of the Protist Helicosporidium sp.
Author(s) -
BOUCIAS DRION G.,
BECNEL JAMES J.,
WHITE SUSAN E.,
BOTT MICHEAL
Publication year - 2001
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2001.tb00180.x
Subject(s) - biology , midgut , galleria mellonella , protist , insect , anopheles albimanus , ultrastructure , microbiology and biotechnology , manduca sexta , botany , anopheles , larva , virulence , biochemistry , malaria , immunology , gene
. We describe the discovery and developmental features of a Helicosporidium sp. isolated from the black fly Simulium jonesi. Morphologically, the helicosporidia are characterized by a distinct cyst stage that encloses three ovoid cells and a single elongate filamentous cell. Bioassays have demonstrated that the cysts of this isolate infect various insect species, including the lepidopterans, Helicoverpa zea, Galleria mellonella , and Manduca sexta , and the dipterans, Musca domestica, Aedes taeniorhynchus, Anopheles albimanus , and An. quadrimaculatus. The cysts attach to the insect peritrophic matrix prior to dehiscence, which releases the filamentous cell and the three ovoid cells. The ovoid cells are short‐lived in the insect gut with infection mediated by the penetration of the filamentous cell into the host. Furthermore, these filamentous cells are covered with projections that anchor them to the midgut lining. Unlike most entomopathogenic protozoa, this Helicosporidium sp. can be propagated in simple nutritional media under defined in vitro conditions, providing a system to conduct detailed analysis of the developmental biology of this poorly known taxon. The morphology and development of the in vitro produced cells are similar to that reported for the achorophyllic algae belonging to the genus Prototheca.