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The SSU rDNA Coding Region of a Filose Amoeba Contains a Group I Intron Lacking the Universally Conserved G at the 3′‐Terminus 1
Author(s) -
BHATTACHARYA DEBASHISH,
OLIVEIRA MARIANA C.
Publication year - 2000
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2000.tb00094.x
Subject(s) - biology , protist , intron , phylogenetic tree , ribosomal rna , ribosomal dna , genetics , amoeba (genus) , coding region , conserved sequence , sequence analysis , microbiology and biotechnology , gene , peptide sequence , botany
. We sequenced small subunit ribosomal DNA (rDNA) PCR‐fragments of sizes 2.3 kb and 2.9 kb isolated from a culture of the red alga, Porphyru spiralis var. spiralis. Phylogenetic analysis of the 2.3‐kb fragment showed that it encoded the sequence of a contaminant filose amoeba. The Nuclearia ‐like amoeba (named strain N‐Por) was identified with scanning electron microscopy. Its rDNA sequence was positioned with strong bootstrap support within a diverse protist assemblage that includes filose amoebae, chlor‐arachniophytes, cercomonads, and Plasmodiophora brussicae. The rDNA of N‐Por contained a group I intron at the conserved 943 position that remarkably, had a U at the 3′‐terminus rather than the universally conserved G.