Premium
Responses of Entamoeba invadens to Heat Shock and Encystation are Related
Author(s) -
FIELD JESSICA,
DELLEN KATRINA,
GHOSH SUDIP K.,
SAMUELSON JOHN
Publication year - 2000
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2000.tb00083.x
Subject(s) - biology , heat shock protein , entamoeba , microbiology and biotechnology , entamoeba histolytica , heat shock , biochemistry , gene
. An Entamoeba invadens gene encoding a homologue of BiP/GRP78, a 70‐kDa heat shock protein or chaperonin was cloned. The predicted E. Invadens BiP contained an ATP‐binding site, a substrate‐recognition domain, and a carboxy‐terminal KDEL‐peptide. Messenger RNAs of E. invadens for BiP, for a 70‐kDa heat shock cognate, for a cyst wall glycoprotein (Jacob), and for chitinase were all induced by heat shock and by encystation medium. The presence of Jacob in heat‐shocked amebae was confirmed by confocal microscopy and suggests that heat shock and encystation responses in E. invadens are related.