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Phylogenetic Analysis of the Small Subunit Ribosomal RNA of Marteilia.refringens Validates the Existence of Phylum Paramyxea (Desportes and Perkins, 1990) 1
Author(s) -
BERTHE FRANCK C. J.,
ROUX FRÉDÉRIQUE,
PEYRETAILLADE ERIC,
PEYRET PIERRE,
RODRIGUEZ DAVID,
GOUY MANOLO,
VIVARÈS CHRISTIAN P.
Publication year - 2000
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2000.tb00049.x
Subject(s) - biology , phylum , ribosomal rna , phylogenetic tree , ribosomal dna , ostrea edulis , 28s ribosomal rna , rna , gene , genetics , oyster , ribosome , ecology
.Marteilia refringens is recognized as one of the most significant pathogens of bivalve molluscs. The nucleotide sequence of the small subunit ribosomal RNA gene of Marteilia refringens is used to elucidate the phylogenetic position of the phylum Paramyxea. Genomic DNA was extracted from sporangia of Marteilia , purified from infected blue mussels, Mytilus edulis , and flat oysters, Ostrea edulis . The sequences obtained from Marteilia species purified from both oysters and mussels were identical. The sequence identity was confirmed by in situ hybridization using a DNA probe targeted to a variable region of the ribosomal DNA. The small subunit ribosomal RNA gene sequence of M. refringens is very different from all known sequences of eukaryotic organisms, including those of myxosporeans and haplosporeans. Therefore, the phylum Paramyxea should continue to be recognized as an independent eukaryotic phylum.