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Ultrastructural Distribution of Poly (A) + RNA During Trypanosoma cruzi‐Cardiomyocyte Interaction in vitro: A Quantitative Analysis of the Total mRNA Content by in situ Hybridization
Author(s) -
PEREIRA MIRIAN CLAUDIA S.,
SINGER ROBERT H.,
MEIRELLES MARIA DE NAZARETH S. L.
Publication year - 2000
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2000.tb00046.x
Subject(s) - trypanosoma cruzi , biology , cytoplasm , rna , intracellular , in situ hybridization , messenger rna , ultrastructure , microbiology and biotechnology , in vitro , kinetoplastida , cell , parasite hosting , biochemistry , anatomy , gene , world wide web , computer science
. Ultrastructural in situ hybridization was used to visualize the spatial distribution of poly (A) + RNA and quantitate its relative amount within different cellular compartments of cardiomyocytes after T. cruzi infection. The amount of poly (A) RNA remained about the same up to 24 h post‐infection. In contrast, its content was reduced 65% after 72 h of interaction, showing a marked decrease in the cell cytoplasm. This decline in poly (A) + RNA level in host cell cytoplasm was concomitant with intracellular proliferation of T. cruzi amastigotes. Thus, T. cruzi may affect host cell cytoplasmic mRNA stability, associated with the parasite's intracellular multiplication.