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Characterization of Sarcocystis falcatula Isolates from the Argentinian Opossum, Didelphis albiventris
Author(s) -
DUBEY J. P.,
LINDSAY D. S.,
VENTURINI L.,
VENTURINI C.
Publication year - 2000
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2000.tb00045.x
Subject(s) - biology , opossum , melopsittacus , didelphis , schizogony , zoology , microbiology and biotechnology , anatomy , immunology , malaria , plasmodium falciparum
. Two isolates of Sarcocystis falcatula were obtained from the lungs of budgerigars ( Melopsittacus undulatus ) fed sporocysts from two naturally‐infected South American opossums ( Didelphis albiventris ). The two isolates were designated SF‐1A and SF‐2A. Both isolates induced fatal infections in budgerigars. Both isolates underwent schizogony in African green monkey kidney cells. The structure of schizonts in the lungs of budgerigars was more variable than that observed in cell culture. The two isolates were identified as 5. falcatula by the two species‐specific Hinf 1 restriction fragments dervied from digestion of a PCR amplification using primers JNB33/JNB54. Thus, the South American opossum, D. albiventris , is a definitive host for S. falcatula .