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Use of Micromanipulation and “Feeder Layers” to Clone the Oyster Pathogen Perkinsus marinus
Author(s) -
BUSHEK DAVID,
HOLLEY RUSSELL A.,
REECE KIMBERLY S.
Publication year - 2000
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2000.tb00027.x
Subject(s) - biology , clone (java method) , oyster , extracellular , pathogen , protozoa , microbiology and biotechnology , ecology , genetics , gene
. Genetic and biochemical characterization of microbes often requires the use of clonal cultures. A method to clone the oyster parasite Perkinsus marinus is described. Individual cells are isolated via micromanipulation and maintained above an actively proliferating “feeder layer” of P. marinus on a 0.45‐μm membrane. Extracellular products released from the proliferating feeder layer can diffuse across the membrane and bathe the isolated cell, stimulating it to proliferate. The method is relatively simple and should be applicable to most protists that can be cultured in the laboratory.