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Recent Advances in the Molecular Genetics of Paramecium 1
Author(s) -
KUNG CHING,
SAIMI YOSHIRO,
HAYNES W. JOHN,
LING KITYIN,
KISSMEHL ROLAND
Publication year - 2000
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2000.tb00003.x
Subject(s) - paramecium , biology , genetics , gene , paramecium caudatum , reverse genetics , gene silencing , complementation , mutant , microbiology and biotechnology
.Paramecium continues to be used to study motility, behavior, exocytosis, and the relationship between the germ and the somatic nuclei. Recent progress in molecular genetics is described. Toward cloning genes that correspond to mutant phenotypes, a method combining complementation with microinjected DNA and library sorting has been used successfully in cloning several novel genes crucial in membrane excitation and in trichocyst discharge. Paramecium transformation en masse has now been shown by using electroporation or bioballistics. Gene silencing has also been discovered in Paramecium , recently. Some 200 Paramecium genes, full length or partial, have already been cloned largely by homology. Generalizing the use of gene silencing and related reverse‐genetic techniques would allow us to correlate these genes with their function in vivo.