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An Indexed Genomic Library for Paramecium Complementation Cloning
Author(s) -
KELLER ANNEMARIE,
COHEN JEAN
Publication year - 2000
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.2000.tb00001.x
Subject(s) - complementation , biology , cloning (programming) , genomic library , genetics , paramecium , library , gene , molecular cloning , computational biology , mutant , microbiology and biotechnology , base sequence , computer science , gene expression , 16s ribosomal rna , programming language
. Recent pioneering work opened the way for cloning genes in Paramecium by functional complementation of mutants. We present here the construction and pilot utilization of a new indexed library of Paramecium macronuclear DNA. The library is made of 61,440 clones containing inserts mostly between 6 and 12 kilobases. It has already allowed the complementation cloning of four new genes, and this library has proven to be very useful for rapid hybridization cloning.