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Characterization of a Sarcocystis neurona. Isolate (SN6) from a Naturally Infected Horse from Oregon
Author(s) -
DUBEY J. P.,
MATTSON D. E.,
SPEER C. A.,
BAKER R. J.,
MULROONEY D. M.,
TORNQUIST S. J.,
HAMIR A. N.,
GERROS T. C.
Publication year - 1999
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1999.tb06067.x
Subject(s) - biology , parasite hosting , horse , spleen , sarcocystis , virology , protozoa , microbiology and biotechnology , immunology , paleontology , world wide web , computer science
An isolate of Sarcocystis neurona. (SN6) was obtained from the spinal cord of a horse from Oregon with neurologic signs. The parasite was isolated in cultures of bovine monocytes and equine spleen cells. The parasite divided by endopolygeny and completed at least one asexual cycle in cell cultures in three days. Two gamma interferon knockout mice inoculated with cell culture‐derived merozoites became ill 35 d later and S. neurona schizonts and merozoites were found in encephalitic lesions. The parasite in tissue sections of mice reacted with S. neurona‐specific antibodies and S. neurona was reisolated from the brain of knockout mice.