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The Dynein Heavy Chain Gene Family In Tetrahymena Thermophila
Author(s) -
XU WENJIE,
ROYALTY MICHAEL P.,
ZIMMERMAN JONELLE R.,
ANGUS STEVEN P.,
PENNOCK DAVID G.
Publication year - 1999
Publication title -
journal of eukaryotic microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.067
H-Index - 77
eISSN - 1550-7408
pISSN - 1066-5234
DOI - 10.1111/j.1550-7408.1999.tb05136.x
Subject(s) - dynein , tetrahymena , biology , flagellum , microtubule , microbiology and biotechnology , dynein atpase , cilium , gene isoform , gene , genetics
The dynein ATPases are a family of motor enzymes that drive microtubule sliding in cilia and flagella and contribute to microtubule‐based transport inside cells. the multi‐dynein hypothesis makes two predictions: 1) Axonemes contain multiple dynein heavy chain (DHC) isoforms, each encoded by a different gene; 2) Each isoform performs a specific role in ciliary beating. We used PCR‐based techniques to clone thirteen different DHC sequences from Tetrahymena genomic DNA. All thirteen genes appeared to be expressed in growing cells. Comparisons of the deduced amino acid sequences of the thirteen DHCs with other known DHCs suggested that we have cloned three outer arm DHCs. two cytoplasmic DHCs, and eight inner arm DHCs.